更新時間：2022-08-11
“人抗透明帶抗體(aZP) "由上海恒遠(yuǎn)生物專業(yè)提供，有96T和48T兩種規(guī)格，包括96/48孔酶標(biāo)包被板、酶標(biāo)試劑、樣品稀釋液、顯色劑AB液、標(biāo)準(zhǔn)品、濃縮洗滌液,封板膜,說明書等。
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企業(yè)單位：上海恒遠(yuǎn)生物
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產(chǎn)品名稱：人抗透明帶抗體（aZP）
英文名稱：Human anti-zona pellucida antibody,aZP ELISA KIT
產(chǎn)品規(guī)格：96人份/48人份
保存條件：2-8℃
產(chǎn)地：美國
實驗方法：酶聯(lián)免疫法/酶免法（ELISA）
人抗透明帶抗體（aZP）檢測原理：采用雙抗體夾心ABC-ELISA原理
| | | | | | |
| 1 | 30倍濃縮洗滌液 | 20ml×1瓶 | 7 | 終止液 | 6ml×1瓶 |
| 2 | 酶標(biāo)試劑 | 6ml×1瓶 | 8 | 標(biāo)準(zhǔn)品（16ng/L） | 0.5ml×1瓶 |
| 3 | 酶標(biāo)包被板 | 12孔×8條 | 9 | 標(biāo)準(zhǔn)品稀釋液 | 1.5ml×1瓶 |
| 4 | 樣品稀釋液 | 6ml×1瓶 | 10 | 說明書 | 1份 |
| 5 | 顯色劑A液 | 6ml×1瓶 | 11 | 封板膜 | 2張 |
| 6 | 顯色劑B液 | 6ml×1/瓶 | 12 | 密封袋 | 1個 |
| 標(biāo)本要求 | | | | | |
| 1．標(biāo)本采集后盡早進(jìn)行提取，提取按相關(guān)文獻(xiàn)進(jìn)行，提取后應(yīng)盡快 | |||||
| 進(jìn)行實驗。若不能馬上進(jìn)行試驗，可將標(biāo)本放于-20℃保存， | | ||||
| 但應(yīng)避免反復(fù)凍融 | | ||||
| 2．不能檢測含NaN3的樣品，因NaN3抑制辣根過氧 | |||||
| 化物酶的（HRP）活性。 | |||||
| Materials provided with the kit | |||||
| 1 | wash solution | 20ml×1bottle | 7 | Stopp Solution | 6ml×1 bottle |
| 2 | HRP-Conjugate reagent | 6ml×1 bottle | 8 | Standard（16pg/ml） | 0.5ml×1 bottle |
| 3 | Microelisa stripplate | 12well×8strips | 9 | Standard diluent | 1.5ml×1bottle |
| 4 | Sample diluent | 6ml×1 bottle | 10 | Instruction | 1 |
| 5 | Chromogen Solution A | 6ml×1 bottle | 11 | Closure plate membrane | 2 |
| 6 | Chromogen Solution B | 6ml×1 bottle | 12 | Sealed bags | 1 |
1. extract as soon as possible after Specimen collection,and according to the relevant literature,
and should be experiment as soon as possible after the extraction. If it can’t, specimen can be
kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
2. Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.
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